UM Directory
Brent Ryckman
Associate Professor
Contact Information
- Department:
- Division Of Biological Sciences
- Email:
- brent.ryckman@umontana.edu
- Phone:
- Office Hours:
-
By appointment.
- Personal Website:
- https://www.umt.edu/ryckman-lab/
Office Address
Division Of Biological Sciences
Interdisciplinary Science Building (ISB) 215
32 Campus Dr MS 4824
Missoula MT, 59812
Interdisciplinary Science Building (ISB) 215
32 Campus Dr MS 4824
Missoula MT, 59812
Courses Taught
1) Virology BIOM 435; 3 CR (Spring semesters)
2) Advanced Virology BIOM 535; 3CR (Spring semesters; concurrent with BIOM435)
3) Topics of Virology BIOB 596; 1CR Spring semesters/co-requisite for BIOM535).
4) Vector-Borne Diseases BIOM 327; 3 CR (Fall semesters)
Research Interests
Molecular biology of human cytomegalovirus (HCMV) replication.
Current research goals:
1) To characterize the glycoprotein complexes present in the HCMV envelope that mediate attachment and entry.
2) To define the mechanisms by which HCMV enters different types of cells.
3) To better understand the specific roles of epithelial and endothelial cells in the pathogenesis of HCMV disease.
Selected Publications
- 2023
- Zehner M, Alt M, Ashurov A, Goldsmith JA, Spies R, Weiler N, Lerma J, Gieselmann L, Stöhr D, Gruell H, Schultz EP, Kreer C, Schlachter L, Janicki H, Laib Sampaio K, Stegmann C, Nemetchek MD, Dähling S, Ullrich L, Dittmer U, Witzke O, Koch M, Ryckman BJ, Lotfi R, McLellan JS, Krawczyk A, Sinzger C, Klein F. Single-cell analysis of memory B cells from top neutralizers reveals multiple sites of vulnerability within HCMV Trimer and Pentamer. Immunity. 2023 Nov 14;56(11):2602-2620.e10. doi: 10.1016/j.immuni.2023.10.009. PubMed PMID: 37967532.
- 2021
- Schultz EP, Yu Q, Stegmann C, Day LZ, Lanchy JM, Ryckman BJ. Mutagenesis of Human Cytomegalovirus Glycoprotein L Disproportionately Disrupts gH/gL/gO over gH/gL/pUL128-131. J Virol. 2021 Aug 10;95(17):e0061221. doi: 10.1128/JVI.00612-21. Epub 2021 Aug 10. PubMed PMID: 34132577; PubMed Central PMCID: PMC8354327.
- 2020
- Day LZ, Stegmann C, Schultz EP, Lanchy JM, Yu Q, Ryckman BJ. Polymorphisms in Human Cytomegalovirus Glycoprotein O (gO) Exert Epistatic Influences on Cell-Free and Cell-to-Cell Spread and Antibody Neutralization on gH Epitopes. J Virol. 2020 Mar 31;94(8). doi: 10.1128/JVI.02051-19. Print 2020 Mar 31. PubMed PMID: 31996433; PubMed Central PMCID: PMC7108825.
- Schultz EP, Lanchy JM, Day LZ, Yu Q, Peterson C, Preece J, Ryckman BJ. Specialization for Cell-Free or Cell-to-Cell Spread of BAC-Cloned Human Cytomegalovirus Strains Is Determined by Factors beyond the UL128-131 and RL13 Loci. J Virol. 2020 Jun 16;94(13). doi: 10.1128/JVI.00034-20. Print 2020 Jun 16. PubMed PMID: 32321807; PubMed Central PMCID: PMC7307150.
- Siddiquey MNA, Schultz EP, Yu Q, Amendola D, Vezzani G, Yu D, Maione D, Lanchy JM, Ryckman BJ, Merola M, Kamil JP. The Human Cytomegalovirus Protein UL116 Interacts with the Viral Endoplasmic-Reticulum-Resident Glycoprotein UL148 and Promotes the Incorporation of gH/gL Complexes into Virions. J Virol. 2021 Jul 12;95(15):e0220720. doi: 10.1128/JVI.02207-20. Epub 2021 Jul 12. PubMed PMID: 34011552; PubMed Central PMCID: PMC8274611.
- 2018
- Zhang L, Zhou M, Stanton R, Kamil J, Ryckman BJ. Expression Levels of Glycoprotein O (gO) Vary between Strains of Human Cytomegalovirus, Influencing the Assembly of gH/gL Complexes and Virion Infectivity. J Virol. 2018 Aug 1;92(15). doi: 10.1128/JVI.00606-18. Print 2018 Aug 1. PubMed PMID: 29743375; PubMed Central PMCID: PMC6052298.
- 2016
- Schultz EP, Lanchy JM, Ellerbeck EE, Ryckman BJ. Scanning Mutagenesis of Human Cytomegalovirus Glycoprotein gH/gL. J Virol. 2015 Dec 9;90(5):2294-305. doi: 10.1128/JVI.01875-15. PubMed PMID: 26656708; PubMed Central PMCID: PMC4810707.
- 2015
- Zhou M, Lanchy JM, Ryckman BJ. Human Cytomegalovirus gH/gL/gO Promotes the Fusion Step of Entry into All Cell Types, whereas gH/gL/UL128-131 Broadens Virus Tropism through a Distinct Mechanism. J Virol. 2015 Sep;89(17):8999-9009. doi: 10.1128/JVI.01325-15. Epub 2015 Jun 17. PubMed PMID: 26085146; PubMed Central PMCID: PMC4524070.
- Li G, Nguyen CC, Ryckman BJ, Britt WJ, Kamil JP. A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4471-6. doi: 10.1073/pnas.1419875112. Epub 2015 Mar 23. PubMed PMID: 25831500; PubMed Central PMCID: PMC4394275.
- 2013
- Zhou M, Yu Q, Wechsler A, Ryckman BJ. Comparative analysis of gO isoforms reveals that strains of human cytomegalovirus differ in the ratio of gH/gL/gO and gH/gL/UL128-131 in the virion envelope. J Virol. 2013 Sep;87(17):9680-90. doi: 10.1128/JVI.01167-13. Epub 2013 Jun 26. PubMed PMID: 23804643; PubMed Central PMCID: PMC3754086.